"This weekend's winter storm is expected to have a significant impact on our operations, particularly at Charlotte International Airport," American said in a statement. It also issued a travel announcement allowing customers affected by the weather to rebook flights free of charge.
Ronald Reagan Washington National Airport is the second most affected airport, with 38 cancellations reported Monday.
A massive storm system hit the eastern United States and the Midwest over the weekend. There were reports of paralyzing ice in parts of Georgia, Virginia and Carolina on Sunday. The governors of these states have declared emergencies.
Monday is Martin Luther King, Jr., day, a federal holiday, and many schools and offices were closed.
--CNN Business' Ramishah Maruf contributed to this report.